Figure 1 | Scientific Reports

Figure 1

From: Bio-Fenton reaction involved in the cleavage of the ethoxylate chain of nonionic surfactants by dihydrolipoamide dehydrogenase from Pseudomonas nitroreducens TX1

Figure 1

(A) 12% SDS-PAGE of the purification steps from P. nitroreducens TX1. Lane 1: Crude cell extract of strain TX1; lane 2: protamine sulfate treated; lane 3: DEAE-Sepharose; lane 4: 25–60% ammonium sulfate; lane 5: Phenyl-Sepharose; lane 6: Sephacryl S-200; lane 7: Mono P HR 5/20; lane M: molecular mass markers. (B) 12% SDS-PAGE of the purification steps from recombinant E. coli BL21 (DE3). Lane 1: Crude cell extract of recombinant cells; lane 2: His-trap; lane 3: Superose 6. The protein sample contains 5 μg in each well. The gel was stained by Coomassie Brilliant blue.

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