Figure 2 | Scientific Reports

Figure 2

From: Bio-Fenton reaction involved in the cleavage of the ethoxylate chain of nonionic surfactants by dihydrolipoamide dehydrogenase from Pseudomonas nitroreducens TX1

Figure 2

Mass spectra of OPEOn degradation by the purified enzyme in the present of metals and NADH. (A) At zero time, the mass spectrum of reaction shows a characteristic pattern of the OPEOn parental ion pattern. The most abundant ethoxymer is OPEO9 (m/z = 625.3). (B) After 2 hours reaction, the profile shifts to lower EO chain products with OPEO6 (m/z = 493.3) as most abundant ethoxymer. The reaction mixture (4 mL) contains 50 mM KH2PO4/Na2HPO4 (pH 8.0), 10 μM MgSO4, 5 μM Co(NO3)2, 100 μM FeSO4, 200 μM ZnSO4, 10 μM CuSO4, 60 μM MnSO4, 40 μM Na2EDTA, 0.05% OPEOn, 96 µg/mL pure Lpd enzyme and 0.5 mM NADH at 30 °C.

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