Figure 4 | Scientific Reports

Figure 4

From: Bio-Fenton reaction involved in the cleavage of the ethoxylate chain of nonionic surfactants by dihydrolipoamide dehydrogenase from Pseudomonas nitroreducens TX1

Figure 4

Degradation of OPEOn by purified recombinant Lpd in the presence of metals and NADH. The reaction mixture (4 mL) contained 50 mM KH2PO4/Na2HPO4 (pH 8.0), 10 μM MgSO4, 5 μM Co(NO3)2, 100 μM FeSO4, 200 μM ZnSO4, 10 μM CuSO4, 60 μM MnSO4, 40 μM Na2EDTA, 0.05% OPEOn, 96 µg/mL pure Lpd enzyme and 0.5 mM NADH at 30 °C. Total ion count of representative OPEOn (n = 5, 7, 9, 11, 13, 15) is expressed as percentage of the initial ion count of the individual ethoxymer according to the function of reaction time. The ethoxymer containing odd numbers of ethoxylate units represents the degradation of each monomer in this figure. Data points and error bars represent the means and standard deviations of triplicate measurements.

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