Figure 6
Secretion of stored chemokines and newly synthesized IL-1β by platelets from patients with dengue or patients coinfected with dengue and HIV: (A–C) Platelets (1 × 109 per mL) obtained at the acute and the recovery phase of dengue illness from 10 patients with dengue and 6 patients with HIV plus dengue coinfection (HIV + dengue) were cultured ex vivo for 4 h. The concentrations of PF4/CXCL4 (A), RANTES/CCL5 (B) and IL-1β (C) in the supernatant of cultured platelets are shown. (D) Freshly isolated platelets from 9 dengue-infected patients, 4 HIV + dengue coinfected patients and 5 age- and sex- matched healthy volunteers were intracellularly labeled for IL-1β. The percentages of platelets expressing IL-1β are shown in each condition. Each dot represents values from one patient or healthy volunteer. The horizontal lines in each group represent the mean. The whiskers in panel D indicate the standard deviation. *Signifies p < 0.05 compared to patients at the recovery phase or to healthy volunteers. #Means p < 0.05 between dengue and HIV + dengue infected patients. ns means nonsignificant.
