Figure 5
From: Reversal of established liver fibrosis by IC-2-engineered mesenchymal stem cell sheets
Comparison of the anti-fibrotic effects of IC-2 sheets between CD90+/CD271+ bone marrow-derived mononuclear cells (BM-MNCs) and adherent BM-MNCs. (A) Micrographs of IC-2 liver sections subjected to Azan staining (upper) and Sirius red staining (lower). (B) Measurement of fibrotic areas based on Azan and Sirius red staining (n = 9–10 except for n = 3 for CCl4(−) group. CCl4(−) indicates control mice without CCl4 intoxication; mean ± S.E.M., *P < 0.05, one-way ANOVA, least significant difference test). (C) Hydroxyproline contents in liver tissues (n = 9–10 except for n = 3 for CCl4(−) group, mean ± S.E.M., *P < 0.05, one-way ANOVA, least significant difference test). (D) MMP-14 activity in BM-MNCs on the eleventh day after IC-2 treatment in vitro (n = 3, mean ± S.D., *P < 0.05, student’s t-test).
