Figure 2 | Scientific Reports

Figure 2

From: ALK4/5-dependent TGF-β signaling contributes to the crosstalk between neurons and microglia following axonal lesion

Figure 2

Molecular characterization of ALK4/5: a TGF-β family type I receptor (TGF-β R1). (a) Nucleotide and amino acid sequences of leech ALK4/5. The numbers of nucleotides are indicated in left and of amino acids in right. The five disulfide bridges are highlighted in dark grey. Two regions highlighted in light grey represent the GS domain and the L45 loop structural motif. (b) Protein sequence showing the preserved pattern in leech ALK4/5. Sequence alignment with human TGF-β type I receptor (ALK5, P36897) and Activin type I receptor (ALK4, P36896). High and low consensus homologies are represented by red and blue residues, respectively. (c–g) Fluorescence in situ hybridization on whole mounted leech CNS 24 h after lesion. Confocal microscopy images show mRNA localization using an antisense alk4/5 riboprobe (green) in the ganglion (c) and in the connective (e) as framed in the CNS diagrams. (f) Arrows show more closely the alk4/5 mRNA positive microglial cells in the point of injury. (d,g) No signal was detected with sense probes used as negative controls. Microglia cell nuclei were stained with Hoechst 33342 (blue).

Back to article page