Figure 5
HO53 and HO56 enhance induction of additional innate immunity genes including cytokines in ALI differentiated BCi cells. Differentiated cells were used for experiments when TEER value reached approx. ≥ 1000 Ω × cm2. Expression at mRNA level of (a) LCN2, (b) S100A8 and (c) subsequent protein level expression of NGAL and S100A8 analyzed by Western blot. GAPDH was used as loading control. The Western blot analysis was performed n = 2 with similar results. Full-length blots are presented in Supplementary Figure S10c. The mRNA level of (d) CAMP, (e) HBD1, (f) LYZ after 24 h (black bars) and 48 h (grey bars) stimulation with HO53 and HO56 (both at 75 μM). Expression at mRNA level was analyzed by qRT-PCR and normalized to TUBB (tubulin-β) reference gene. Data is from n = 4 independent experiments ± SEM, statistical significance was calculated in comparison to untreated cells (Ctrl) using two-way ANOVA with Dunnett’s multiple comparisons test, *p < 0.05, **p < 0.01, ***p < 0.001. Significant changes are indicated.
