Figure 4
Residues that contribute substantially to interactions in Gαq complexes with RGS2/8, PLC-β3, p63RhoGEF, and GRK2. (a) Gαq residues that substantially contribute to interactions with RGS2 and RGS8. The Gαq-RGS2/8 crystal structures (PDB IDs: 4EKD and 5DO9) were superimposed using the Gαq coordinates. (b) RGS2 and RGS8 residues that substantially contribute to interactions with Gαq. The crystal structures of Gαq-RGS2 (PDB ID: 4EKD) and Gαq-RGS8 (PDB ID: 5DO9) were superimposed using RGS coordinates, shown as gray ribbons. (c) Gαq residues that contribute substantially to interactions with PLC-β3 (PDB ID: 3OHM). (d) PLC-β3 (maroon ribbon) residues that contribute substantially to interactions with Gαq. (e) Gαq residues that contribute substantially to interactions with p63RhoGEF (PDB ID: 2RGN). (f) p63RhoGEF (cyan ribbon) residues that contribute substantially to interactions with Gαq. (g) Gαq residues that contribute substantially to interactions with GRK2 (PDB ID: 2BCJ). (h) GRK2 (pink ribbon) residues that contribute substantially to interactions with Gαq. In all panels, residues that contribute substantially to interactions with the cognate partner are shown as spheres and colored according to the type of energy contribution: side-chain polar/electrostatic and non-polar contributions, magenta; side-chain polar/electrostatic contribution only, red; main-chain polar/electrostatic contribution only, yellow; main-chain polar/electrostatic and non-polar contributions, blue; non-polar contributions only, green. In panels a,c,e, and g – Gαq is shown as a gold ribbon and the cognate partner as a transparent gray molecular surface. In panels b,d,f, and h – Gαq is shown as a transparent gray molecular surface.
