Table 1 Oligonucleotide sequences used in the study.
From: Proteomic and structural differences in lumpfish skin among the dorsal, caudal and ventral regions
Gene name | Primer sequence | Amplicon size | Primer efficiency (%) | Purpose of use |
|---|---|---|---|---|
apoA1 | F: TACMTRRCTCRGGTGAARGASA | 528 | — | Degenerate PCR |
R: CTTGTAYTCYKSARCRTAGGG | ||||
F: ACATGCACACCAAGCTCAG | 107 | 98.678 | qPCR | |
R: AATGATTGAGGAGCGGAAG | ||||
calm | F: CAGATTGCHGARTTYAARGARGC | 392 | — | Degenerate PCR |
R: GTTGACCTGDCCRTCWCCRT | ||||
F: ACGGACAGTGAGGAGGAGA | 110 | 87.770 | qPCR | |
R: TTCTCCCCGAGGTTAGTCA | ||||
hspa8 | F: GGCACTACCTACTCCTGTGTAG | 706 | — | Degenerate PCR |
R: TTTGAACTCRGAGATGAAGTGG | ||||
F: TCTCATTGGACGTCGGTTT | 119 | 101.439 | qPCR | |
R: TGGTCTCGCCCTTGTACTC | ||||
hist1h2b | F: ACCAGGAAGGAGAGCTATGCYATC | 268 | — | Degenerate PCR |
R: CTTGGTGACGGCCTTKGTDC | ||||
F: ATCTTTGAGCGCATCGCCG | 144 | 104.115 | qPCR | |
R: TGTTCCCTCAGACACCGCG | ||||
gapdh | F: GCCATCAAYGACCCMTTCAT | 380 | — | Degenerate PCR |
R: GCAGTTRGTVGTGCAGGADG | ||||
F: GGGGCAAGCTCATCGTCG | 149 | 104.03 | qPCR | |
R: CCTGGATGTGAGAGGAGGCC | ||||
β-actin | F: GACTACCTCATGAAGATCCTGA | 188 | qPCR | |
R: GGTGATGACCTGTCCGTC | ||||
ef1-alfa | F: AAGTTCGAGAAGGAAGCCGC | 98 | 100.584 | qPCR |
R: ATGGTGATACCACGCTCACG | ||||
pentraxin | F: ATCATCCTGTTYGCSTAYCGAC | Did not show amplification | ||
R: GTCCCACAWKTKCASVTYWGHMA | ||||
natterin-2 | F: GCAGAGACCTGGACCAAGACGT | Did not show amplification | ||
R:GCTTCTCCCTTCTATGGTACCC | ||||
