Figure 1 | Scientific Reports

Figure 1

From: The Rho/MRTF pathway inhibitor CCG-222740 reduces stellate cell activation and modulates immune cell populations in KrasG12D; Pdx1-Cre (KC) mice

Figure 1

Treatment with the Rho/MRTF pathway inhibitor CCG-222740 prevents the activation of primary stellate cells. Stellate cells isolated from the pancreas of wildtype mice were treated with CCG-222740 at 1 μM for 6 days. (A) Bright field microscopy of the stellate cells; arrows point to fibers and arrowheads to duplicating cells (100x magnification). (B) Stellate cell activation was evaluated by measuring the levels of alpha smooth muscle actin (α-SMA) and collagen 2 A (COL2A) by Western blotting. (C) Levels of α-SMA were normalized to vinculin and quantified using ImageJ. Data are represented as mean ± SEM. Blots are representative of 3 independent experiments. Additional blots in Supplemental Fig. 8. *p < 0.05.

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