Figure 4
Example of unsupervised clustering (VISp area M248L1). Panels (A,B) show the distribution of the clusters as direct output of the original clustering algorithm and panels (C,D) the respective layers after 7th and 8th iterations. Panels (F,G) represent the whole iteration process and the hierarchical tree obtained with neuron density as unique feature (panel E). The manually labelled layers are indicated to the right of panel (F). Panel (H) shows a corresponding x–z slice, and bottom panels seven x-y slice examples at depth z = 40 µm (I), z = 160 µm (J), z = 280 µm (K), z = 320 µm (L) z = 460 µm (M), z = 620 µm (N), and z = 690 µm (O), corresponding to the seven c-layers on the 8th hierarchical level of panel (F).
