Figure 5

(A) Thickness of actin fibers in Mel270 and BLM cells treated with low-LET proton beam radiation or X-rays. Measurements of 15 cells in each experimental group were taken at 20 and 40 days following radiation. Two types of fibers were measured: marginal and internal also called stress fibers with 15 measurements for each type of fibers per cell. Results are presented as the percent of the untreated control. Mean values ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. (B) Representative images of actin cytoskeleton stained in Mel270 and BLM cells after treatment with low-LET proton beam radiation or X-rays. Cells were stained with phalloidin conjugated with Alexa 546. Scale bar: 10 μm.