Figure 6
From: Bile-duct proliferation as an unexpected side-effect after AAV2-LDLR gene transfer to rabbit liver
Immunohistochemical characterization of representative rabbit liver samples after AAV mediated gene transfers. (a–d) Representative images of Ki-67 staining for proliferating cells in liver sections 1 year after AAV mediated gene transfer of LDLR or control. (e–h) Immunohistochemical staining for pan-cytokeratin stained both normal small and large bile ducts (e,g,h) and the aberrant tubules in the areas with bile duct proliferation (f). (i,j) β-galactosidase immunostaining of AAV2-control liver 1 month after gene transfer revealed positive staining in hepatocytes (arrow), inflammatory cells (arrowhead) and biliary epithelium (asterisk). Magnification 200x, inserts 400x. Scale bar: 100 µm.
