Figure 5

Analysis of neo-glomerular filtration. We injected low-molecular-weight, tetramethylrhodamine-labelled dextran into host rats. The accumulation of dextran in Bowman’s space of GFP-positive glomeruli (arrowhead) and in the lumen of GFP-positive tubules (arrow) was confirmed by immunostaining, demonstrating glomerular filtration (scale bars, left column: 50 µm, right upper and right lower column: 10 µm). Representative images of two specimens from one transplantation experiment are indicated.