Figure 2 | Scientific Reports

Figure 2

From: Acute-on-chronic liver disease enhances phenylephrine-induced endothelial nitric oxide release in rat mesenteric resistance arteries through enhanced PKA, PI3K/AKT and cGMP signalling pathways

Figure 2

Effect of endothelial denudation on phenylephrine-induced vasoconstriction in mesenteric resistance arteries from Sham-Operated (SO, A) and microsurgical liver cholestasis (LC, B) rats. Results (mean ± S.E.M.) were expressed as a percentage of the initial contraction elicited by KCl. n = 10 animals per experimental group. *P < 0.05 (Bonferroni post-hoc test). (C) Differences of area under the curve (in percentage) in the absence or presence of endothelium. (D) Western blot analysis for alpha-1 adrenoceptors in mesenteric resistance arteries from SO and LC rats. Each lane is representative of 8 isolated arterial segments from different animals in each group. A rat brain homogenate was used as a positive control (+C). Lower panel shows the densitometric analysis for the alpha-1 adrenoceptor expression. Results (mean ± S.E.M.) were expressed as the relation between the signal obtained for the protein analysed and the signal obtained for β-actin.

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