Table 3 PCR programs and primers set used for detection of HCV, parvovirus b19 and HBV.

From: First Report of Prevalence of Blood-Borne Viruses (HBV, HCV, HIV, HTLV-1 and Parvovirus B19) Among Hemophilia Patients in Afghanistan

Target

Sequence

PCR Conditions

Parvo virus B19

F1: GGTTGATTATGTGTGGG

R1: ACTGAAGTCATGCTTGG

F2: TGTGTGTTGTGTGCAAC

R2: CAAACTTCCTTGAAAATG

Step

Temp

Time

35 cycles

Initial Denaturation

94 °C

5 min

Denaturation

94 °C

1 min

Annealing

55 °C

1.5 min

Extension

72 °C

2 min

Final Extension

72 °C

10 min

HCV

F1:GAAAGCGTCTAGCCATGGCGTTAGT

R1: CTCGCAAGCACCCTATCAGG

Step

Temp

Time

45 cycles

Initial Denaturation

94 °C

5 min

Denaturation

94 °C

15 Sec

Annealing

56 °C

30 Sec

Extension

72 °C

1 min

Final Extension

72 °C

10 min

HBV

F1: AGAACATCGCATCAGGACTC

R1: CATAGGTATCTTGCGAAAGC

F2: AGGACCCCTGCTCGTGTTAC

R2: AGATGATGGGATGGGAATAC

Step

Temp

Time

40 cycles

Initial Denaturation

94 °C

5 min

Denaturation

94 °C

1 min

Annealing

55 °C

1 min

Extension

72 °C

2 min

Final Extension

72 °C

10 min

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