Table 1 Primers used for measuring the genetic expression of Nrf2, NF-κB, caspase-3 and GAPDH.

From: Thymoquinone therapy remediates elevated brain tissue inflammatory mediators induced by chronic administration of food preservatives

Gene

Forward Primer

Reverse Primer

Nrf2

5′-GAGACGGCCATGACTGAT-3′

5′-GTGAGGGGATCGATGAGTAA-3′

NF-κB

5′-ACCCTGACCTTGCCTATTTG-3′

5′-GAAAAGCTGTAAACATGAGCCG-3′

caspase-3

5′-CGTCTGTGCTCCAGGCTTC-3′

5′-TGTGAGTTCCTTCCTTTCTTTGTG-3′

GAPDH

5′-CCATCAACGACCCCTTCATT-3′

5′-CACGACATACTCAGCACCAGC-3′

Back to article page