Table 2 Kinetic parameters of EcHpaB and its variants toward different substrates.
| Â | p-Coumaric acid | Umbelliferone | Resveratrol | Naringenin | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Km μM | kcat min−1 | kcat/Km s−1mM−1 | Km μM | kcat min−1 | kcat/Km s−1mM−1 | Km μM | kcat min−1 | kcat/Km s−1mM−1 | Km μM | kcat min−1 | kcat/Km s−1mM−1 | |
WT | 137.6 ± 21.0 | 23.2 ± 0.7 | 2.8 | 217.0 ± 60.6 | 25.1 ± 2.4 | 1.9 | 174.3 ± 17.9 | 26.2 ± 0.6 | 2.5 | 349.8 ± 77.6 | 9.0 ± 0.3 | 0.4 |
XS2 | 387.9 ± 32.7 | 11.0 ± 0.4 | 0.47 | 490.8 ± 17.3 | 16.9 ± 2.6 | 0.6 | 404.6 ± 93.8 | 24.3 ± 2.4 | 1.0 | 1061.7 ± 21.1 | 6.5 ± 0.3 | 0.1 |
XS3 | 235.6 ± 40.2 | 29.8 ± 1.3 | 2.1 | 266.2 ± 41.1 | 14.8 ± 0.6 | 0.9 | 235.8 ± 52.8 | 33.9 ± 2.8 | 2.4 | 417.2 ± 10.2 | 9.0 ± 0.4 | 0.4 |
XS4 | 210.8 ± 85.3 | 30.7 ± 3.7 | 2.4 | 204.5 ± 16.3 | 22.3 ± 0.5 | 1.8 | 441.8 ± 22.1 | 50.8 ± 8.8 | 1.9 | 627.5 ± 75.0 | 6.1 ± 0.1 | 0.2 |
XS5 | 235.3 ± 52.5 | 22.5 ± 1.3 | 1.56 | 346.4 ± 11.2 | 13.0 ± 1.6 | 0.6 | 319.8 ± 9.9 | 20.0 ± 2.4 | 1.0 | 661.0 ± 93 | 7.8 ± 0.2 | 0.2 |
XS6 | 132.1 ± 29.1 | 21.9 ± 1.0 | 2.8 | 176.9 ± 35.9 | 20.9 ± 1.4 | 2.0 | 144.0 ± 22.9 | 25.0 ± 1.2 | 2.9 | 191.6 ± 33.6 | 9.0 ± 0.2 | 0.8 |
