Figure 5
pUL97-inflicted developmental defects and mortality are kinase-dependent. (a) Single cell-staged embryos were injected with plasmids encoding active pUL97-FLAG, the inactive mutant pUL97(K335M)-FLAG or empty vector (75 ng/µl). At 24 hpf, embryos were categorized in phenotype classes as described in Fig. 2a and quantitated. (b) Western blot analysis using total lysates of 24 hpf zebrafish embryos (~6 embryos per lane) injected with diluent or plasmids as indicated. Blots were probed with anti-FLAG and anti-α-tubulin antibodies. (c) Single cell-staged embryos were injected with the indicated expression plasmids or empty vector pcDNA3.1 (75 ng/µl). Subsequently embryos were raised until 24 hpf in the presence or absence of 1 µM MBV and phenotypes were quantitated. For each condition and independent experiment 55 to 151 (a) and 19 to 36 (b) embryos were analyzed (in total 851 (a) or 673 (b) embryos in 3 independent experiments). Data are mean ± SD. ***p < 0.01.
