Figure 5

CAY10594 elicits therapeutic effects on APAP-induced acute liver injury. (A) After fasting for 16 h, APAP (500 mg/kg) was administered to mice. Either the vehicle (PBS containing 1% DMSO) or CAY10594 (8 mg/kg) was administered to the APAP-challenged mice at 3 h post APAP. The livers were isolated from the APAP-challenged mice at 12 h, and stained with hematoxylin and eosin (magnification, x100, x200) (A). The data are representative of two independent experiments with individual samples. Each experiment was performed in quintuplicates. Scale bar: 200 μm (A). (B) Survival rate was monitored from mice which were intraperitoneally injected with either the vehicle (PBS containing 1% DMSO) or CAY10594 (8 mg/kg) at 3 h after the APAP challenge (750 mg/kg). The survival rate was monitored every 6 h for 72 h. *P < 0.05 by log-rank test. Sample size: n = 8 (B).