Figure 3

(A) Left: ESS spectra were obtained from a series of locations from outer surface of kidneys after removal of Gerota’s fascia. Right: The kidney was then sliced open (bisected), and ESS spectra were obtained from various locations of the cortex and medulla as shown. (B) Averaged spectra obtained from the inside (cortex and medulla) and surface of obstructed left kidney over four weeks duration are shown. Four animals per time point after induction of UUO model are compared with kidneys from control animals. The spectra are normalized at 650 nm to facilitate comparison of spectral shapes and relative spectral amplitudes at the shorter wavelengths.