Figure 3
(A) HEK-293 cells, transfected with HA-JNK1α2, HA-JNK2α2, or HA-JNK3α2 and STX1a (left blots), were IP for HA and probed for HA, STX1a and JNK2 (right blot) after 40 μM JGRi1 or sJGRi1 treatment (right blots). Cropped WBs are here shown. (B) p-JNK IP experiment. JNK phosphorylation was augmented by 10 min NMDA (100 μM) + glycine (1 μM) with respect to not stimulated control, while it was reduced by 30 min pretreatment with JGRi1 (1 μM). The scrambled peptide failed to diminish JNK phosphorylation, demonstrating no effect in reducing the effects of NMDA stimulation. Mg2+-free medium has been used during NMDA stimulus. JGRi1 reduced the levels of STX1a, whereas the scrambled version did not. Experiment run in triplicate. Cropped WBs are here shown.
