Figure 2 | Scientific Reports

Figure 2

From: Activation of Th lymphocytes alters pattern expression and cellular location of VIP receptors in healthy donors and early arthritis patients

Figure 2

Cellular localization of VPAC receptors during the activation of memory Th lymphocytes from HD. (A) Immunofluorescence analysis on resting- (upper) and seven days activated- Th cells (lower) using specific antibodies for VPAC1 (Alexa Fluor 488, green), and VPAC2 (Alexa Fluor 594, red). Nuclei were counterstained with Hoechst (Blue). Fluorescence was examined on Leica SP2 AOBS confocal microscope (63X). Original scale bars, 20 µm. Boxed areas show higher magnification views of individual cells (3,17x zoom). Results are representative of five different donors. (B) VPAC1 and VPAC2 receptors in plasma membranes and nuclear fraction of resting- and seven days activated- memory Th cells were measured by Western blotting. Na+/K+ ATPase protein levels were determined as plasma membrane marker. Three of five different donors were shown.

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