Figure 3

Distribution analysis of VPAC receptors across the Th lymphocytes from HD. Zoomed 63x images were imported into ImageJ to analyze the distribution of VIP receptors across the cell. (A) Representative graphs of intensity profiles plotted as a function of distance (measured in pixels) versus intensity (measured in gray-scale values) showed a distinctive distribution of receptors on each cell states. (B) Orthogonal projections of merged images from resting (up) and activated cells (down). In the lower side: XZ plane; in the right side: YZ plane.