Figure 6 | Scientific Reports

Figure 6

From: Activation of Th lymphocytes alters pattern expression and cellular location of VIP receptors in healthy donors and early arthritis patients

Figure 6

CD30 expression is increased by VIP by both receptors in activated memory Th cells from healthy donors. (A) Expression of CD30 was determined by flow cytometry in activated Th cells at day 4. Auto-fluorescence and isotype controls were set up to determine the non-specific fluorescence signal and percentage of total CD30 positive cells was quantified. The indicated proportion of positive cells was determined in the gate of CD4+CD45RO+ cells (a representative dot plot is shown). Comparison of CD30 Geometric Mean fluorescence intensity (gMFI) in the different conditions is shown. The values of gMFI was corrected by the percentage of CD30+ cells in each condition. Data are the mean ± SEM of five different donors performed by duplicated (*p < 0.05). (B) Soluble CD30 was analysed in culture supernatants by ELISA in activated Th cells at day 4. Results are the mean ± SEM of duplicate determinations of six different HD samples (**p < 0.01).

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