Figure 7

VIP and its receptors maintain the migration capacity of activated memory Th cells from healthy donors. Expression of CCR6, CCR7, CXCR3 and CXCR4 was determined by flow cytometry in resting and one day activated Th cells. Auto-fluorescence and isotype controls were set up to determine the non-specific fluorescence signal and percentage of total positive cells was quantified. Results represent the mean ± SEM of duplicate determinations of five different HD samples (*p < 0.05, **p < 0.01, ***p < 0.001).