Figure 5
From: Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice
EMT in BSA-induced damaged tubule was associated with increased levels of DPP-4, integrin β1 and CAV1; TENE treatment ameliorated these alterations. (a–e) Multiplex immunofluorescence microscopy analysis of the EMT program and association with CAV1. Formaldehyde-fixed, paraffin-embedded (FFPE) kidney samples were labeled with epithelial markers for E-cadherin, αSMA and CAV1. An immunofluorescence analysis was performed by confocal microscopy. (d) The enlarged image of the inset shown in (c). The αSMA-positive damaged tubular cells were surrounded by αSMA-positive interstitial cells (f–j). Multiplex immunofluorescence was performed to analyze the crosstalk among DPP-4, integrin β1 and CAV1 in the BSA-injected diabetic mice. (i) The enlarged image of the inset shown in (h). DPP-4, integrin β1, and CAV1 were localized at the same location (likely the luminal side of the proximal tubule). The crosstalk occurred more frequently in the damaged tubular cells. Representative images from n = 7 in each group are shown.
