Table 1 The optimal collision energies (CEs) and selected transitions for alkylated hepcidin-25 (light and heavy versions).

From: A Targeted Mass Spectrometric Assay for Reliable Sensitive Hepcidin Quantification

Compound name

Precursor ion5+

Product ion

Collision energy

Fragment

Optimized SRM method and selected target compounds.

DTHFPICIFCCGCCHRSKCGMCCKT, M 3251.259

651.259

501.209

22

[b4]+

651.259

756.943

12

[y17] 3+

651.259

794.637

11

[y18] 3+

651.259

847.981

11

[y19]3+

DTHFPICIFCCGCCHRSKCGMCCKT, M 3259.971

652.862

501.209

22

[b4]+

652.862

759.614

12

[y17] 3+

652.862

797.309

11

[y18] 3+

652.862

850.652

11

[y19]3+

  1. C = C[+57.0], K = K[13C6,15N2], Ions chosen as quantifiers in bold, the others were used as qualifiers.
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