Figure 1

Camp and mCRAMP expression is significantly increased in the mouse uterus after 1 µg and 20 µg intrauterine LPS injection. Real-time PCR analysis of relative Camp mRNA expression after 1 µg (a) or 20 µg (d) intrauterine LPS or PBS injection. Relative mCRAMP protein levels after 1 µg (b) or 20 µg (e) intrauterine LPS or PBS injection as quantified by Western Blot analysis (c, f). mCRAMP protein levels were normalised against housekeeping alpha-Tubulin (50 kDa). The mCRAMP protein band is indicated by a black arrow at approximately 18 kDa. Unpaired t-test (*p < 0.05, ****p = 0.0001). Data presented as mean ± SD. The full-length blots with mCRAMP expression are presented in Supplementary Figs S3 and S4.