Figure 3 | Scientific Reports

Figure 3

From: Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Figure 3

LAMP primer combinations with JOE labelled FIP. LAMP amplification and fluorescent detection of 50000 copies per 5 microlitres linear plasmid DNA using 35Sp primers; (A) 0.8 micromolar JOE-FIP with 1x other primers (0.8 micromolar BIP, 0.4 micromolar LoopF and LoopB, 0.2 micromolar F3 and B3), (B) 0.4 micromolar JOE-FIP, 0.4 micromolar FIP, 1x other primers, (C) 0.2 micromolar JOE-FIP, 0.6 micromolar FIP, 1x other primers, (D) no fluorescently labelled primers, 1x primers, (E) 0.4 micromolar JOE-FIP, 1x other primers, (F) 1.2 micromolar JOE-FIP, 1x other primers, (G) 1.6 micromolar JOE-FIP, 1x other primers, (H) 1.6 micromolar JOE-FIP, 1.6 micromolar BIP, 1x other primers, (I) 1.6 micromolar JOE-FIP, 2x other primers. Red lines: no template control; blue lines: samples with 50000 copies of target.

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