Figure 4 | Scientific Reports

Figure 4

From: Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Figure 4

Detection with JOE-FIP and SYTO9. LAMP amplification with 35S promoter primers of a linearised plasmid template (1) quenched fluorescence in presence of JOE-FIP primer with SYTO9 using yellow channel detection of JOE, (2) fluorescence of same samples with green channel detection of SYTO9, (3) fluorescence with unmodified primers with SYTO9 only (green channel). (A) Normalised fluorescence truncated from 60 minute assay, (B) melt curve analysis, (C) average Ct plotted against template concentration on a log scale and (D) summary of replicate Ct values, average Ct and standard deviation (SD) for each dilution. NTCs (red lines) were negative. Light blue lines: 500000 copies; orange: 50000 copies; dark blue: 5000 copies; brown: 500 copies; green: 50 copies.

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