Figure 7 | Scientific Reports

Figure 7

From: Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Figure 7

Detection of LAMP amplification with SYTO9 and JOE-FIP reporting compared to LAMP-BART results. Panels (1A) to (1F) represent amplification with the 35S promoter primers of a low concentration of denatured maize genomic DNA (0.1 percent maize GM event Bt11). Panels (2A) to (2F) represent amplification of the same sample with the NOS terminator primers. Panels (1A and 2A) show LAMP amplification with BART detection with corresponding frequency distribution of positive results (1B and 2B). Panels (1C and 2C) show detection using SYTO9 and (1E and 2E) show the simultaneous results from JOE-FIP detection. The melt curve analysis for these results are shown in panels (1D,1F,2D,2F).

Back to article page