Figure 9 | Scientific Reports

Figure 9

From: Reduced False Positives and Improved Reporting of Loop-Mediated Isothermal Amplification using Quenched Fluorescent Primers

Figure 9

Duplexed LAMP reaction with ADH1 and 35Sp detection. LAMP amplification and detection of 20 copies of denatured Bt11 maize genomic DNA with ADH1 LAMP primers with FAM labelled FIP and 35Sp LAMP primers with TAMRA labelled FIP. Blue indicates template amplification and orange denotes the NTCs. The (DUPLEX) column shows the detection of two LAMP reactions in each tube. Other columns show the individual LAMP reactions and the detection channels of the thermocycler; FAM-FIP detected on green channel only and TAMRA-FIP by the orange channel.

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