Table 1 Oligonucleotide primers for LAMP. Primers targetting the 35S promoter, NOS terminator and the ADH1 reference gene have been described previously and are attributed to Guy Kiddle (GK)9 and David Lee (DL)52.
Target, Type, Notation | Length | Primer Sequence (5′ to 3′) |
|---|---|---|
35Sp, Displacement, F3 | 20 | CTTATATAAGAGGAAGGGTCT |
35Sp, Displacement, B3(P) | 21 | GATAAAGGAAAGGCTATCATT |
35Sp, Displacement, B3(G) | 20 | ATAAAGGAAAGGCCATCGTT |
35Sp, LAMP, FIP | 39 | CCACGTCTTCAAAGCAAGTGG-TTTT-GGATAGTGGGATTGTGCGTC |
35Sp, LAMP, JOE-FIP | 45 | [JOE]CCACGTCTTCAAAGCAAGTGG-TTTT-GGATAGTGGGATTGTGCGTC |
35Sp, LAMP, TAMRA-FIP | 45 | [TAMRA]CCACGTCTTCAAAGCAAGTGG-TTTT-GGATAGTGGGATTGTGCGTC |
35Sp, LAMP, BIP | 37 | TTCCACGATGCTCCTCG-TTTT-CCTCTGCCGACAGTGG |
35Sp, Loop, LoopF | 16 | TCCACTGACGTAAGGG |
35Sp, Loop, FAM-LoopF | 16 | [FAM]TCCACTGACGTAAGGG |
35Sp, Loop, LoopB | 16 | GGGGTCCATCTTTGGG |
NOSt, Displacement, F3 | 22 | CGCGATAATTTATCCTAGTTTG |
NOSt, Displacement, B3 | 19 | CGTTCAAACATTTGGCAAT |
NOSt, LAMP, FIP | 46 | GCATGACGTTATTTATGAGA-TTTT-TCGCGCTATATTTTGTTTTCTA |
NOSt, LAMP, JOE-FIP | 46 | [JOE]GCATGACGTTATTTATGAGA-TTTT-TCGCGCTATATTTTGTTTTCTA |
NOSt, LAMP, BIP | 43 | CATGCTTAACGTAATTCAACA-TTTT-TGAATCCTGTTGCCGGTC |
NOSt, Loop, LoopF | 22 | GATTAGAGTCCCGCAATTATAC |
NOSt, Loop, LoopB | 23 | AAATTATATGATAATCATCGCAA |
ADH1, Displacement, F3 | 19 | CTTTGGATCGATTGGTTTC |
ADH1, Displacement, B3 | 17 | CCCAAAATTACTCAACG |
ADH1, LAMP, FIP | 48 | CCCTCCGCAAATCTTCGAAC-TTTT-GTAACTGGTGAGGGACTGAG |
ADH1, LAMP, FAM-FIP | 48 | [FAM]CCCTCCGCAAATCTTCGAAC-TTTT-GTAACTGGTGAGGGACTGAG |
ADH1, LAMP, BIP | 50 | GGTGATCAAGTGCAAAGGTC-TTTT-CATAAACCAAGATTAGTCAGATCAAG |
ADH1, Loop, LoopF | 20 | CGCCTTGTTTCTCCTCTGTC |
ADH1, Loop, LoopB | 16 | CAATCCACTCCGAGAC |
