Figure 3

Cellular localization of PgCad1 proteins within Hi5 cells. Hi5 cells transfected with pIE2-sPgCad1-GFP (a–d), pIE2-r15APgCad1-GFP (e–h), or pIE2-r15BPgCad1-GFP (i–l). Nuclei stained with Hoechst 3342 are shown in blue, dsRED-labeled endoplasmic reticulum shown in red, and GFP-labeled PgCad1 fusion proteins are shown in green. Superimposed images from (a–c) are shown in (d), from (e–g) in (h), and from (i–k) in (l). The arrow in (d) indicates cell membrane. Bar = 20 μm.