Figure 6

Inhibition of foam cell formation by atorvastatin. (a) Effect of different concentration of atorvastatin on foam cell formation. Oil Red O staining was performed to identify the foam cell formation under the different concentration of the drug. The images were obtained by a Leica microscope at × 20 bright field. (b) Effect of different concentration of atorvastatin on ROS level. (c) Statistical analysis of the lipid droplets generation for (a) experiment. (d) Statistical analysis of intracellular ROS level for (b) experiment. The total green fluorescence intensity of each well was quantified by using a fluorescence multi-well plate reader. (e) The mRNA expression of the related factors in the co-culture model under different conditions. The method was the same as in Fig. 5a. (f) The protein expression of the related factors in the co-culture model under different conditions. The method was the same as in Fig. 5b. The data were shown as mean ± SD (*p < 0.05, **p < 0.01).