Table 2 Hydrodynamic parameters of PTPN3-PDZ derived from the analysis of analytical ultracentrifugation.

From: Structural and functional characterization of the PDZ domain of the human phosphatase PTPN3 and its interaction with the human papillomavirus E6 oncoprotein

PTPN3-PDZ

 

10 μM

25 μM

70 μM

70 μM + HBVc PBM

  

MW theoretical (kDa)

10.5

10.5

10.5

12.0

  

MW estimated (kDa)

12.4

11.3–25.9a

nc

13.5

  

Sed coef (S0,w,20) (S)

1.5

1.5–2.6a

nc

1.6

  

PTPN3-PDZ Next

 

17.5 μM

35 μM

70 μM

70 μM + HPV16E6 PBM

70 μM + HPV18E6 PBM

70 μM + HBVc PBM

MW theoretical (kDa)

12.7

12.7

12.7

14.1

14.1

14.2

MW estimated (kDa)

12.7

12.5

15

13.7

14.7

15.6

Sed coef (S0,w,20) (S)

1.6

1.7

1.6

1.6

1.6

1.6

  1. aTwo peaks are present; nc: not calculated because of a continuum of species.
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