Figure 1
From: Affimer reagents as tools in diagnosing plant virus diseases
Wild type CPMV and CPMV eVLP have the same antigenicity. (a) Overlay of published CPMV structures, 5a33, 5a32, 5FMO, 5MS1, 5MSH. The RMSD between these structures is 0.4. The Small (S) subunit is coloured blue and the Large (L) subunit is coloured green. (b) Coomassie blue-stained SDS-PAGE gel to show the total protein concentration of CPMV samples compared with a western blot which demonstrates that a polyclonal antiserum raised against WT CPMV detects both the L and S subunits of WT CPMV-T, M and B and eVLP-CPMV with the same efficiency. (c) Phage ELISA of Affimer proteins from 24 clones incubated in wells containing immobilised empty virus like particles (eVLPs) (pink), WT CPMV (blue) and a negative control (green), showing the 3,3′,5,5′-tetramethylbenzidine (TMB) product absorbance at 620 nm after 2 minutes. Clones labelled with a black asterisk (*) were selected as Affimer proteins suitable for detecting both eVLP and WT CPMV and were sequenced. Clones labelled with a red asterisk were identified as Affimer proteins with different sequences at the variable loops and used for further testing.
