Figure 3
From: Affimer reagents as tools in diagnosing plant virus diseases
Anti-CPMV Affimer 3 binds to CPMV at the 2-fold axis. (a) 3D cryo-EM reconstruction of CPMV bound to anti-CPMV Affimer 3. The CPMV S subunit is coloured blue and the L subunit is green. Extra EM density attributed to anti-CPMV Affimer 3 is shown in purple. Icosahedral (I1) symmetry was imposed during image processing. The 2-fold axis is indicated with a dashed red line. (b) Zoomed in view of bound anti-CPMV Affimer 3 (purple) side and top views. The crystal structure of an Affimer (PDB 4N6U) is fitted into the density. As anti-CPMV Affimer 3 is bound to the 2-fold axis, density is not visualized for the beta strands due to averaging required for image processing. The sharpened map contains high resolution information and only the alpha helical portion of the Affimer can be visualized. The unsharpened map contains low resolution information where density for the beta strands can be visualized. The 2-fold axis is indicated with a dashed red line.
