Figure 4
From: Affimer reagents as tools in diagnosing plant virus diseases
Anti-CPMV Affimer 3 is specific to CPMV. (a) ELISA of immobilized VLPs - Murine Norovirus (MNV), Satellite Tobacco Necrosis Virus (STNV), Hepatitis B (Hep B) and Potato Leaf Roll Virus (PLRV) detection using anti-CPMV Affimer 3. Only CPMV and eVLP-CPMV were detected by anti-CPMV Affimer 3. (b) A schematic describing a sandwich ELISA. Anti-CPMV Affimer 3 is immobilized on a maxisorb plate, if CPMV is present it binds to anti-CPMV Affimer 3. Biotinylated anti-CPMV Affimer 3 binds to the CPMV capsid (if present) which then binds to streptavidin-HRP. HRP oxidises TMB to produce a colour change which is monitored by absorbance. (c) A sandwich ELISA of a blind test to detect the presence of CPMV in infected leaves. 6 leaves were a negative control and 6 leaves were infected with CPMV (diluted to either 1 in 5 or 1 in 20). Leaf extract numbers 2, 4, 5, 6, 9 and 11 were positive. These crude leaf extracts also showed increased absorbance demonstrating the detection method was successful. Error bars show standard deviation from the mean.
