Figure 2
From: Analysis of extracellular vesicles generated from monocytes under conditions of lytic cell death
THP-1 monocytes release EVs following treatment with primary necrotic, secondary necrotic, pyroptotic and apoptotic stimuli. NTA analysis of cell-free supernatants isolated following 300 g and subsequent 2000 g centrifugation, collected from THP-1 cells following hyperthermic stress (A–C), 24 h UV irradiation (D–F), LPS + nigericin treatment (or LPS alone) (G–I) or 4 h UV irradiation (J–L), compared with supernatants from untreated control THP-1 cells. Histograms in (A,D,G,J) represent particle concentration vs size distribution. Bar graphs in (B,E,H,K) illustrate mean particles concentrations for each of the conditions. Mean and mode EV size for each of the conditions are presented as bar graphs in (C,F,I,L). Data are representative of three independent experiments. Unpaired, two-tailed student t-tests were performed, *P < 0.05, ***P < 0.001. Error bars = S.E.M. (N = 3).
