Figure 8
Effects of mycalolide A on SRF-responsive transcription by the luciferase reporter assay. SRF-responsive luciferase activity was measured in HeLa cells treated with the indicated concentrations of mycalolide A (92% purity), cytochalasin D, and latrunculin B for 3 hr. After normalizing the activities with co-transfected Renilla luciferase, the relative values to that in cells treated with DMSO (the solvent) were obtained. Data are expressed as means ± SD (from 3 independent experiments), where asterisks indicate a statistically significant difference (**p ≤ 0.01; *p ≤ 0.05). In 300 nM mycalolide A, many dead cells were observed and so the value at this condition (#) should not be directly compared with the others.
