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Figure 2

From: Identification and first characterization of DinJ-YafQ toxin-antitoxin systems in Lactobacillus species of biotechnological interest

Figure 2

Identification and sequence alignment of dinJ-yafQ TA systems in wild Lactobacillus strains. (a) Outcome of PCR-based screening on wild Lactobacillus strains: (+) positive strains, (−) negative strains. (b) Sequence alignment of YafQ toxins identified in L. casei, L. paracasei and L. rhamnosus wild strains and compared with E. coli YafQ (UniProt Q47149). Catalytic residues are highlighted in green. (c) Sequence alignment of DinJ antitoxins identified in L. casei, L. paracasei and L. rhamnosus wild strains and compared with E. coli DinJ (UniProt Q47150). (d) Structure representation and close view of E. coli K-12 YafQ active sites (orange; PDB ID: 4ml2) and homology models of YafQ_pa4366 and YafQ_rh2360 (grey) with predicted catalytic residues shown in sticks. (e) Front and rear views of YafQ_rh2360 homology model with the N-terminal region lacking in YafQ_rh1473 highlighted in red. Catalytic histidines are shown in sticks.

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