Figure 3 | Scientific Reports

Figure 3

From: Comprehensive transcriptome analysis and functional characterization of PR-5 for its involvement in tomato Sw-7 resistance to tomato spotted wilt tospovirus

Figure 3

Functional validation of a candidate resistance gene PR-5 (OLP). (A) PR-5 gene was inserted between 35S and NOS terminator with an N-terminus FLAG tag. Transgenic plant lines regenerated on media containing phosphinothricin, but non-transformed plants could not survive (Top left). (B) PCR confirmation showed the presence of transgene (PR-5) and RT-PCR revealing the expression of transgene (PR-5) with positive (+), negative (−) controls and control (GFP) plants. Actin serves as the internal control in both cases for PCR reactions. (C) Percent of test plants infected as evaluated weekly over five weeks post inoculation on transgenic plants expressing OLP-PR5 gene, transgenic plants expressing GFP, and non-transformed ‘Moneymaker’ plants. (D) Evaluation of transgenic lines with resistance to TSWV: PR-5 over-expressing line (OLP-PR5) showed resistance to TSWV with no visible symptoms, whereas control plants (non-transformed Moneymaker) displayed chlorosis and necrotic spots upon TSWV inoculation.

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