Table 3 HPLC-MSn information on phenolic compounds and hydroxy fatty acids identification in D. indicum var. aromaticum flower.

From: An Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower

Peak No.

Rt (min)

UV λmax (nm)

[M-H]−

MSn m/z (% base peak)

Identification

1

3.59

260

499

MS2[499]: 481 (30), 353 (10), 191 (100), 173 (60)

3-O- Caffeoyl-5-O-p- coumaroylquinic acid

2

10.64

230, 300

685

MS2[685]: 539 (100), 523 (60), 665 (35); MS3[539]: 377 (100)

Coumaroyloleuropein

3

11.17

250, 340

447

MS2[447]: 285 (100)

Luteolin-O-glucoside

4

11.81

250, 330

533

MS2[533]: 353 (100), 335 (5), 191 (8), 173 (2); MS3[353]: 309 (1), 191 (100), 179 (16), 173 (6), 135 (4)

3,5-Dicaffeoylquinic acid monohydrate

5

11.92

240, 330

515

MS2[515]: 353 (100), 335 (5), 191 (4); MS3[353]: 191 (100), 179 (40), 173 (10), 135 (7)

3,5-Dicaffeoylquinic acid

6

12.30

250, 270, 320

187

MS2[187]: 169 (6), 125 (100)

Gallic acid monohydrate

7

13.05

250, 290

173

MS2[173]: 146 (8), 131 (100), 127 (15)

Shikimic acid isomer

8

13.32

250, 270, 320

569

MS2[569]: 551 (2), 525 (100), 459 (4), 417 (20), 391 (10); MS3[525]: 507 (16), 482 (18), 427 (10), 379 (28), 235 (15), 193 (100)

Methyoxyoleuropein isomer

9

13.99

250, 350

285

MS2[285]: 241 (35), 217 (20), 199 (25), 175 (25)

Luteolin

10

14.68

300

453

MS2[453]: 435 (2), 411 (6), 393 (100); MS3[393]: 363 (20), 249 (30), 231 (100), 205 (35); MS4[231]: 187 (100)

Prenyl-dimethoxy- caffeoyl-p-coumaric acid

11

16.41

270, 330

269

MS2[269]: 225 (40), 201 (12), 149 (10)

Apigenin

12

17.05

250, 330

299

MS2[299]: 284 (100); MS3[284]: 256 (100), 227 (10), 212 (5)

Chrysoeriol

13

17.49

250, 270, 340

329

MS2[329]: 314 (100); MS3[314]: 299 (100), 285 (10)

Tricin

14

19.74

260

659

MS2[659]: 615 (100), 591 (1), 573 (1), 505 (1), 265 (2); MS3[615]: 573 (100)

Unknown

15

21.30

240, 310

293

MS2[293]: 275 (100), 265 (25), 231 (45), 205 (40), 249 (90), 193 (28), 163 (20)

Monohydroxy- octadecaditrienoic acid

16

23.33

250, 310

/a

/a

Unknown

17

24.73

270, 330

283

MS2[283]: 269 (100)

Acacetin

18

26.61

270, 330, 350

/a

/a

Unknown

19

26.96

270, 310

313

MS2[313]: 295 (100), 277 (45), 215 (10), 183 (18)

Dihydroxy- octadecenoic acid

20

27.33

270, 320, 350

309

MS2[309]: 291 (50), 265 (10), 247 (100)

Dihydroxy- octadecatrienoic acid I

21

27.54

270, 320

309

MS2[309]: 291 (100), 265 (32), 247 (30)

Dihydroxy- octadecatrienoic acid II

22

28.58

310

295

MS2[295]: 277 (100), 265 (30)

Monohydroxy- octadecadienoic acid

23

28.91

270, 320, 350

297

MS2[297]: 279 (100), 251 (65), 223 (20)

Monohydroxy- octadecenoic acid

  1. aNo data available.
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