Table 4 Results for method validation.

From: An Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower

No.

Standard

Linear regression curve

R2

LOD (μg/mL)

LOQ (μg/mL)

Recovery rate (%)a

RSD (%)b

Intra-day

Inter-day

1

Gallic acid

Y = 18817X − 10155

0.9996

0.014

0.042

/c

/c

/c

2

Caffeic acid

Y = 43686X − 19547

0.9998

0.010

0.030

/c

/c

/c

3

Apigenin

Y = 29655X + 6320.6

0.9999

0.013

0.040

99.50 ± 2.69

1.16

2.33

4

Acacetin

Y = 26463X + 9248.4

0.9999

0.013

0.041

102.19 ± 3.10

0.68

1.54

5

Luteolin

Y = 24041X + 44178

0.9989

0.036

0.109

100.47 ± 2.96

1.84

2.70

  1. aRecovery rate (%) = [(measured amount after spiking − measured amount before spiking)/actual amount spiked] * 100.
  2. bRSD (%) = (SD/Mean) * 100, where RSD means relative standard deviation and SD means standard deviation.
  3. cNot analyzed.
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