Table 1 Primers used in this study.
Name | Sequence 5′–3′ |
|---|---|
p1_sgRNA_nour_1 | TAATACGACTCACTATAGGGTACCGCACCAGTGTCCCG |
p2_sgRNA_nour_1 | TTCTAGCTCTAAAACCGGGACACTGGTGCGGTACC |
p1_sgRNA_nour_2 | TAATACGACTCACTATAGGTAAGCCGTGTCGTCAAGAG |
p2_sgRNA_nour_2 | TTCTAGCTCTAAAACCTCTTGACGACACGGCTTAC |
p1_sgRNA_hom2_1 | TAATACGACTCACTATAGGGGACCTGTTGCGGATGCGC |
p2_sgRNA_hom2_1 | TTCTAGCTCTAAAACGCGCATCCGCAACAGGTCCC |
p1_sgRNA_hom2_2 | TAATACGACTCACTATAGGGCTGGGAAAGAGCGTGGGT |
p2_sgRNA_hom2_2 | TTCTAGCTCTAAAACACCCACGCTCTTTCCCAGCC |
hom2_1000_fw | AGGCCTCAGAGGTAGAGAAGC |
hom2_1000_rv | GTACCCCACTGTACCGCCTA |
hom2_750_fw | TGTGCGGCGCATCATATC |
hom2_750_rv | TCGCTGCTGCATACAGTTTA |
hom2_500_fw | CGGATAGGTGCATGGAAGTT |
hom2_500_rv | CACCTCTCGCTTATCGGAAG |
hom2_250_fw | GCGAAAAGACCGGACTCG |
hom2_250_rv | ATCCTAGGAAGCCGGAAGC |
hom2_100_fw | ATCGAAGTGAAATCGGGAAC |
hom2_100_rv | TCGGGCTGCTATCACTCTG |
hom2_ChkA | AAGAGCCCCATGTTCAAG |
hom2_ChkD | CGCGGATAGCAGTTTATCG |
