Figure 5 | Scientific Reports

Figure 5

From: Establishment and genomic characterization of gingivobuccal carcinoma cell lines with smokeless tobacco associated genetic alterations and oncogenic PIK3CA mutation

Figure 5

Mutational profile and validation of RNA-seq data. (A) Somatic nonsynonymous variants in genes recurrently mutated in ≥10% ICGC GB-SCC or TCGA HNSCC samples. (B) Somatic truncating and splice site mutations and missense variants predicted to be deleterious by ≥5 functional prediction tools. *Variants classified as deleterious by 4 prediction tools. (C–E) Validation of RNA-seq data by qPCR. 30 genes identified to be differentially expressed across OSCC cell lines were validated by Real time qRT-PCR. Comparison of gene expression values of RNA-seq and qPCR showed concordance in the data.

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