Table 1 Primers used for amplification and sequencing of the isolate Ych.
From: Phenotypic and genetic characterisation of an emerging reovirus from Pekin ducks in China
Gene segment | Primer name | Sequence (5′ → 3′) | Location | Product size (bp) |
|---|---|---|---|---|
S1 | Ych-S1F | GCATGCAATGGTGGTACAGTG | 35–55 | 1506 |
Ych-S1R | CTTACTGCGTGACATGGACC | 1521–1540 | ||
S2 | Ych-S2F | GTACGAGTTTTTCTCTGTGCC | 30–50 | 1238 |
Ych-S2R | CCTAATTGGTGAAAGTGGCC | 1248–1267 | ||
S3 | Ych-S3F | CAATGGAGGTGCGTATGCC | 29–47 | 1049 |
Ych-S3R | CTGAAGGTAGTGGGTCGTGTC | 1057–1077 | ||
S4 | Ych-S4F | CAACACTTCTGCTGCTGCCG | 56–75 | 1055 |
Ych-S4R | GGGAAACAGACAATAAGACG | 1091–1110 | ||
M1 | Ych-M1-1F | CTATCTAGCCACACCCGTG | 18–36 | 1313 |
Ych-M1-1R | CGTCACTATCCATAATAGTG | 1311–1330 | ||
Ych-M1-2F | GATATCAGATGCTTCGGGAAG | 1074–1094 | 965 | |
Ych-M1-2R | CAGCTACGATGCGAAATTCG | 2019–2038 | ||
M2 | Ych-M2-1F | TATCGCTCACCATGGGCAAC | 20–39 | 1187 |
Ych-M2-1R | CTCATTCGGATTGAACGAGCC | 1186–1206 | ||
Ych-M2-2F | CCTCGCACTTACAACATCCG | 973–992 | 1145 | |
Ych-M2-2R | CTGGCGTGGATTCAGCTTAAC | 2097–2117 | ||
M3 | Ych-M3-1F | ATGATGGCGTCCACTAAGTGG | 22–42 | 1388 |
Ych-M3-1R | CGATTCATACGTTGCAGATCC | 1389–1409 | ||
Ych-M3-2F | CAGATATGGTAGCGTGTCGAC | 1242–1262 | 730 | |
Ych-M3-2R | CGTCCATGATCCACGTTGAG | 1952–1971 | ||
L1 | Ych-L1-1F | GCTCCAGTTTCTGAGAAGAAAG | 60–81 | 1249 |
Ych-L1-1R | CTCTCGAGGGATACATGACC | 1289–1308 | ||
Ych-L1-2F | TCTTGAGCAACTTGCACCTC | 1165–1184 | 1292 | |
Ych-L1-2R | CATCGACGCTCTAATCGATTG | 2436–2456 | ||
Ych-L1-3F | CCTATACGGATCACTAATCCG | 2316–2336 | 1589 | |
Ych-L1-3R | CAATCGATTAGAGCGTCGATG | 3885–3904 | ||
L2 | Ych-L2-1F | CAGTCAAAGGTGTTTTGGCC | 19–38 | 1337 |
Ych-L2-1R | CTGAAATAGAGGGTCCCAAG | 1336–1355 | ||
Ych-L2-2F | CACCCTATTGGTTCCTTACG | 1227–1246 | 1336 | |
Ych-L2-2R | CTGAATAGGCTAGAGAAAGC | 2543–2562 | ||
Ych-L2-3F | GTGATCGCTTGGAGATGTGG | 2434–2453 | 1324 | |
Ych-L2-3R | CGCACAAAGTTCTGCATTCC | 3738–3757 | ||
L3 | Ych-L3-1F | CAGATTCGAGGTTTGCGCTTG | 19–39 | 1344 |
Ych-L3-1R | CAAGTCCAATGGATAACCAGC | 1342–1362 | ||
Ych-L3-2F | ATTCCCTTTGCTGGCATGC | 1218–1236 | 1400 | |
Ych-L3-2R | GGTAGTCCAACTGCATGTAG | 2598–2617 | ||
Ych-L3-3F | CTTCCACTGGCTGGATTGTG | 2466–2485 | 1375 | |
Ych-L3-3R | TGGAGGCACGTAGAAAGACG | 3821–3840 |
