Figure 1
From: Regulation of Cholesterol Homeostasis by a Novel Long Non-coding RNA LASER
Identification of LASER in hepatocyte cell lines, and the association of LASER with cholesterol levels in PBMCs from patients. (A) The expressions of AP000770.1-1, AP000770.1-2 and NR_037884.1 in different cell lines were determined by semi-quantitative RT-PCR. The amplified products were visualized following 2% agarose gel electrophoresis. To verify the specificity of RT-PCR, one negative control is shown without reverse transcriptase. (B) LASER expression in PBMCs of the hypercholesterolemia (n = 26) and normal cholesterol subgroups (n = 149). Data are compared with the Mann–Whitney U test. (C–E) The Spearman correlation of LASER expression in PBMCs with the levels of total cholesterol (C), LDL-c (D) and apoB100 (E) in statin free individuals respectively (n = 175).
